| Solution Information | help | |
| Enzyme: | Genome polyprotein | |
| inhibitor: | BDBM39346 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | NS2bNS3 HTS Assay Protocol. Aprotinin, Sigma, A-4529. Brij-35 solution, 30%, Sigma, B4184-1L Dimethylsulfoxide, (DMSO) Sigma, 154938. Glycerol, Sigma, G5516-1L. Pyr-RTKR-AMC Substrate, BaChem I-1650. Tris Base, Molecular Biology Grade, EMD Biosciences, 648310. Consumables 384-well black low-volume assay plate, Greiner 784076. 384-well low profile pyramid bottom reservoir, Innovative Microplate S30022 PK384 Robotic Tip series, 30uL Axygen, PK-384-R Preparation of Stock Solutions: Aprotinin, 1 mM: To make 1 mM Aprotinin dissolve 6.51 mg Aprotinin in 1 mL 20% glycerol. Aliquot and store at -20#C. Aprotinin Inhibitor Solution: Prepare Fresh Daily 100 nM: Dilute .5 uL 1 mM Aprotinin and 150uL DMSO in 4.85 mL 20% glycerol. 3uM : Dilute 3uL 1mM Aprotinin and 30uL DMSO in 967uL 20% glycerol. 4uM : Dilute 4uL 1mM Aprotinin and 30uL DMSO in 967uL 20% glycerol. 10X Assay Buffer: 100 mM Tris, pH8.0, 0.05% Brij-35: 100 | |
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